Sains Malaysiana 52(11)(2023): 3075-3090
http://doi.org/10.17576/jsm-2023-5211-05
Seaweed-Based Dripping Stimulates Root Growth of Dragon Fruit (Hylocereus
undatus [Haw.] Britton & Rose) Stem Cuttings
(Titisan Berasaskan Rumpai Laut
Merangsang Pertumbuhan Akar Keratan Batang Buah Naga (Hylocereus undatus [Haw.] Britton & Rose))
MAYALENE M. POTENTE1 & BRYL I. MANIGO2,*
1Institute of Agriculture and Related Sciences, Davao del Sur State
College, Digos City, Davao del Sur 8002, Philippines
2Department of Agricultural Sciences, University of Southeastern
Philippines, Tagum-Mabini Campus, Apokon, Tagum City, Davao del Norte 8100,
Philippines
Diserahkan: 30 Mei 2023/Diterima:
19 Oktober 2023
Abstract
Developing a solid root system is essential in
dragon fruit propagation not only for securing the succulent stem but also for
facilitating the absorption of water and essential minerals from the soil. However, dragon fruit stem cuttings normally
develop good root system for about 4-6 months after planting. There are
commercially available growth hormones which hasten rooting, but these are
relatively expensive. Hence, alternative hormones from natural materials that
possess the ability to stimulate rooting are extensively explored. The efficacy
of seaweed-based drippings (SD) as potential rooting bio-stimulant for dragon
fruit cuttings were evaluated under nursery condition. Branch cuttings (30 cm long) were soaked at 6, 12 and 18 h at the
rate of 50 mL, 100 mL and 150 mL SD per liter of water (H2O), with H2O, and ANAA (5
mL/L of H2O) served as positive and negative control treatments, respectively. There were 18 cuttings in each experimental units,
replicated thrice and arranged in a 3 × 5 Completely Randomized Design (CRD).
Results showed that 12 h soaking initiated more and longer roots
and shoots. In addition, it appeared that the application of a solution
containing SD at 100 mL/L of H20 resulted in the development of
numerous and longer roots and shoots, a larger number of lateral roots, and a
greater root-shoot ratio than other treatments. Furthermore, application of SD
at 100 mL/L of H20 through dipping for 12 h developed
more lateral roots compared to other treatment combinations. In conclusion, it can be inferred that the
presence of phytohormones, such as auxins, in seaweed concentrates has the
potential to effectively stimulate root formation in pitaya.
Keywords: Asexual propagation; branch cuttings; pitaya; rooting hormone; seaweed drippings
Abstrak
Mewujudkan sistem akar yang kukuh adalah penting dalam pembiakan buah
naga bukan sahaja untuk menjamin batang sukulen tetapi juga untuk memudahkan
penyerapan air dan mineral penting daripada tanah. Walau bagaimanapun, keratan
batang buah naga biasanya membentuk sistem akar yang baik selama 4-6 bulan
selepas ditanam. Terdapat hormon pertumbuhan yang boleh didapati secara
komersial yang mempercepatkan pengakaran, tetapi ini agak mahal. Oleh itu,
hormon alternatif daripada bahan semula jadi yang mempunyai keupayaan untuk
merangsang perakaran diterokai secara meluas. Keberkesanan titisan berasaskan
rumpai laut (SD) sebagai bio-stimulan pengakaran yang berpotensi untuk keratan
buah naga dinilai di peringkat nurseri. Keratan batang (30 cm panjang) direndam
selama 6, 12 dan 18 jam pada kadar SD 50 mL, 100 mL dan 150 mL bagi setiap
liter air (H2O) dengan H2O dan ANAA (5 mL/L H2O)
masing-masing berfungsi sebagai rawatan kawalan positif dan negatif. Terdapat
18 keratan dalam setiap unit uji kaji, diulang tiga kali dan disusun dalam Reka
Bentuk Rawak Penuh (CRD) 3 × 5. Keputusan menunjukkan bahawa 12 jam perendaman
mengeluarkan akar dan pucuk yang lebih banyak dan panjang. Selain itu,
penggunaan larutan yang mengandungi SD pada 100 mL/L H20
menghasilkan akar dan pucuk yang banyak dan lebih panjang, bilangan akar
lateral yang lebih banyak dan nisbah pucuk-akar yang lebih tinggi daripada
rawatan lain. Tambahan pula, penggunaan SD pada 100 mL/L H20 melalui
pencelupan selama 12 jam menghasilkan lebih banyak akar lateral berbanding
gabungan rawatan lain. Sebagai kesimpulan, boleh disimpulkan bahawa kehadiran
fitohormon, seperti auksin dalam pati rumpui laut mempunyai potensi berkesan
untuk merangsang pembentukan akar dalam pitaya.
Kata kunci: Hormon pengakaran; keratan
batang; pembiakan aseks; pitaya; titisan rumpai laut
RUJUKAN
Abd El-Mohdy,
H.L. 2017. Radiation-induced degradation of sodium alginate and its plant
growth promotion effect. Arabian Journal of Chemistry 10:
S431-S438.
Anderson, G.D. 2023. Kelp extracts as
biostimulants: An investigation of when and why they work. University of
Adelaide. PhD Dissertation (Unpublished).
Andrade, R.A.D., Oliveira, I.V.D.M. & Martins, A.B.G. 2005. Influence of the
condition and storage period in germination of red pitaya seeds. Revista
Brasileira de Fruticultura 27: 168-170.
Atzmon, N. &
van Staden, J. 1994. The effect of seaweed concentrates on the growth of Pinus
pinea seedlings. New Forests 8(3): 279-288.
Chen, D., Zhou, W., Yang, J., Ao, J.,
Huang, Y., Shen, D. & Shen, H. 2021.
Effects of seaweed extracts on the growth, physiological activity, cane yield
and sucrose content of sugarcane in China. Frontiers in Plant Science 12: 659130.
Crane, J.H. & Balerdi, C.F.
2004. Pitaya Growing in Florida Landscape. University of Florida,
Institute of Food and Agricultural Sciences.
Crouch, I.J. & van Staden, J. 1993. Evidence for the
presence of plant growth regulators in commercial seaweed products. Plant
Growth Regulator 13(1): 21-29. doi: 10.1007/BF00207588
Crouch, I.J.,
Beckett, R.P. & van Staden, J. 1990. Effect of seaweed concentrate on the
growth and mineral nutrition of nutrient-stressed lettuce. Journal of
Applied Phycology 2(3): 269-272.
Crouch, I.J.,
Smith, M.T., van Staden, J., Lewis, M.J. & Hoad, G.V. 1992. Identification
of auxins in a commercial seaweed concentrate. Journal of Plant Physiology 139(5):
590-594.
Dachlan, A., Syam’un, E., Asrul,
L., Ulfa, F. & Irindu, D. 2020. Growth of red dragon fruit seedlings (Hylocereus
costaricensis L.) from two sources of cuttings at various concentrations of
shallot solutions. In IOP Conference Series: Earth and Environmental
Science 486(1): 012112. IOP Publishing.
Dessalegn, Y. & Reddy, Y.N. 2003. Effects of different concentrations of
auxins on rooting and root characters of air and ground layers of jojoba (Simmondsia
chinensis (Link.) C.K. Schneider. Ethiop. J. Sci. 26(2): 155-159.
DOST-PCAARRD Department of Science
and Technology- Philippine Council for Agriculture, Aquatic and Natural
Resources Research and Development. 2016. First dragon fruit festival
in the Philippines held.
http://www.pcaarrd.dost.gov.ph/home/portal/index.php/quick-informationdispatch/1940-first-dragon-fruit-festival-in-the-philippines-held. Accessed on February 19, 2020.
Du Jardin, P. 2015. Plant biostimulants:
Definition, concept, main categories and regulation. Sci. Hortic. 196:
3-14. 10.1016/j.scienta.2015.09.021
Ecosystems Research and
Development Bureau. 2013. Dragon Fruit and Palawan Cherry. Laguna: Research
Information Series on Ecosystems. 25(1): January-April.
Eusebio, J.E.
& Alaban, M.C.S. 2018. Current status of dragon fruit and its prospects in
the Philippines. Country Report, Dragon Fruit Regional Network Initiation
Workshop.
Fitter,
A. 2002. Characteristics and functions of root systems. In Plant Roots: The
Hidden Half, 3rd ed., edited by Waisel, Y., Eshel, A., Beeckman, T. &
Kafkafi, U. Boca Raton: CRC Press. pp. 49-78.
Garande, V.K., Gawade, M.H., Sapkal, R.T. & Gurav, S.B. 2002.
Efect of IBA and number of internodes on rooting of stem cuttings of grape
rootstocks. Agricultural Science Digest 22(3): 176-178.
Genard, H. 1991.
Effect of salinity on lipid composition, glycine betaine content and
photosynthetic activity in chloroplasts of Suaeda maritima. Plant
Physiol. Biochem. 29: 421-427.
Hardjadinata, S.
2010. Budi Daya Buah Naga Super Red Secara Organik. Jakarta:
Penebar Swadaya.
Hobbie, L. 2003. Auxin. In Encyclopedia
of Hormones, edited by Henry, H.L. & Norman, A.W. Massachusetts:
Academic Press pp. 186-197.
Jeannin, I.,
Lescure, J.C. & Morot-Gaudry, J.F. 1991. The effects of aqueous seaweed
sprays on the growth of maize. Botanica Marina 34(6): 469-474.
Khan, W.,
Rayirath, U.P., Subramanian, S., Jithesh, M.N., Rayorath, P., Hodges, D.M.
& Prithiviraj, B. 2009. Seaweed extracts as biostimulants of plant growth
and development. Journal of Plant Growth Regulation 28(4):
386-399.
Krajnc, A.U., Turinel, M. &
Ivancic, A. 2013. Morphological and physiological changes during adventitious
root formation as acted by auxin metabolism: Stimulatory effect of auxin
containing seaweed extract treatment. Agricultura 10(1-2): 17-27.
Landi, M., Zivcak, M., Sytar, O.,
Brestic, M. & Allakhverdiev, S.I. 2020. Plasticity of photosynthetic
processes and the accumulation of secondary metabolites in plants in response
to monochromatic light environments: A review. Biochimica et Biophysica Acta
(BBA)-Bioenergetics 1861(2): 148131.
La Torre, A., Battaglia, V. & Caradonia, F. 2016. An overview of
the current plant biostimulant legislations in different European Member
States. Journal of the Science of Food and Agriculture 96(3):
727-734.
Le Bellec, F.
& Judith, R.C. 2002. La pitaya (Hylocereus sp.) en culture de
diversification à l'île de la Réunion: stratégie d'accompagnement de son
développement. Fruits 57(4): 219-230.
Madende, M. & Hayes, M. 2002.
Fish by-product use as biostimulants: An overview of the current state of the
art, including relevant legislation and regulations within the EU and USA. Molecules 25(5): 1122.
Mancuso, S.,
Azzarello, E., Mugnai, S. & Briand, X. 2006. Marine bioactive substances
(IPA extract) improve foliar ion uptake and water stress tolerance in potted Vitis
vinifera plants. Advances in Horticultural Sciences 20(2):
156-161.
Mori, C.V., Patel, A.R., Parmar, V.K. & Patel, G.S. 2023. Dragon fruit (Kamalam): An
excellent exotic fruit crop of India. The Pharma Innovation International
Journal 12(1): 115-123.
Mukherjee, A. & Patel, J.S. 2020. Seaweed extract:
Biostimulator of plant defense and plant productivity. International Journal
of Environmental Science and Technology 17: 553-558.
Nabati, D.A.,
Schmidt, R.E. & Parrish, D.J. 1991. Effect of a PGR product and Fe on
water-stressed Kentucky bluegrass. In Proceedings of the Plant Growth
Regulator Society of America (USA).
Nanda, K.K. 1975.
Physiology of adventitious root formation. Indian Journal of Plant
Physiology 18: 80-89.
Nguyen, T.D., Venkatadri, U., Nguyen-Quang,
T., Diallo, C. & Adams, M. 2019. Optimization model for fresh fruit supply
chains: Case-study of dragon fruit in Vietnam. AgriEngineering 2(1):
1-26.
Nelson, W.R.
& van Staden, J. 1986. Effect of seaweed concentrate on the growth of
wheat. South African Journal of Science 82: 199-200.
Ordög, V., Stirk,
W.A., van Staden, J., Novák, O. & Strnad, M. 2004. Endogenous cytokinins in
three genera of microalgae from the Chlorophyta 1. Journal of Phycology 40(1):
88-95.
Panda, D.,
Pramanik, K. & Nayak, B.R. 2012. Use of seaweed extracts as plant growth
regulators for sustainable agriculture. International journal of
Bio-resource and Stress Management 3(3): 404-411.
Paradiso, R. & Proietti, S. 2022. Light-quality
manipulation to control plant growth and photomorphogenesis in greenhouse
horticulture: The state of the art and the opportunities of modern LED systems. Journal of Plant Growth Regulation 41(2): 742-780.
Patier, P., Yvin,
J.C., Kloareg, B., Liénart, Y. & Rochas, C. 1993. Seaweed liquid fertilizer
from Ascophyllum nodosum contains elicitors of plantD-glycanases. Journal
of Applied Phycology 5(3): 343-349.
Patil, V.N., Chauhan, P.S., Panchabhai, D.M., Shivankar,
R.S. & Tannirawar, A.V. 2000. Effect of growth regulators on rooting of hardwood cuttings of some commercial
grape varieties. J. Soils and Crops 10(2): 295-297.
Poorter, H., Niklas, K.J., Reich, P.B., Oleksyn, J., Poot,
P. & Mommer, L. 2012. Biomass allocation to leaves, stems and roots:
meta-analyses of interspecific variation and environmental control. New
Phytol. 193: 30-50. doi: 10.1111/j.1469-8137.2011.03952.x
Pramanick, B.,
Brahmachari, K., Ghosh, A. & Zodape, S. T. 2016. Effect of seaweed saps
derived from two marine algae Kappaphycus and Gracilaria on
growth and yield improvement of blackgram. Indian Journal of Geo-Marine
Sciences 45(6): 789-794.
Philippine Statistics Authority
(PSA). 2018. Volume of Production and Area Planted for Dragon Fruit in the
Philippines (2012-2017). Crops Statistics Division, Economic Sector
Statistics Service, Sectoral Statistics Office, Quezon City, Philippines.
Rajan, R.P. & Singh, G. 2021. A
review on the use of organic rooting substances for propagation of horticulture
crops. Plant Archives 21(1): 685-692.
Rathore, S.S.,
Chaudhary, D.R., Boricha, G.N., Ghosh, A., Bhatt, B.P., Zodape, S.T. &
Patolia, J.S. 2009. Effect of seaweed extract on the growth, yield and nutrient
uptake of soybean (Glycine max) under rainfed conditions. South
African Journal of Botany 75(2): 351-355.
Santos Teixeira, J. A., & Ten Tusscher, K. H. 2019. The systems biology of lateral root formation: connecting the dots. Molecular Plant 12(6): 784-803.
Schmidt, R.E.,
Ervin, E.H. & Zhang, X. 2003. Questions and answers about
biostimulants. Golf Course Manage 71(6): 91-94.
Seran, T.M. &
Thiresh, A. 2015. Root and shoot growth of dragon fruit (Hylocereus undatus)
stem cutting as influenced by indole butyric acid. Agricultural and
Biological Sciences Journal 1(2): 27-30.
Shah, S., Li, X., Jiang, Z., Fahad, S.
& Hassan, S. 2022. Exploration of the phytohormone regulation of energy
storage compound accumulation in microalgae. Food and Energy Security 11(4): e418.
Shehata, S.M.,
Abdel-Azem, H.S., Abou El-Yazied, A. & El-Gizawy, A.M. 2011. Effect of
foliar spraying with amino acids and seaweed extract on growth chemical
constitutes, yield and its quality of celeriac plant. European Journal
of Scientific Research 58(2): 257-265.
Shield, P. 2012. Three
Organic Alternatives to Hormone Rooting Powder. http://montrouchorganic.over-blog.com/article-three-organicalternatives-to-hormone-rooting-powder-101172112.html
Siddiqua, A.,
Thippesha, D., Shivakumar, B.S., Adivappar, N. & Ganapathi,
M. 2018. Effect of growth regulators on rooting and shooting of stem cuttings
in dragon fruit [Hylocereus undatus (Haworth) Britton & rose]. Journal
of Pharmacognosy and Phytochemistry 7(5): 1595-1598.
Singh, H.P. 2010. Quality seed and planting material as a
key to success for horticultural development in the country. Progressive
Horticulture 42(1): 1-14.
Singh, K.K. & Chauhan, J.S. 2020. A
review on vegetative propagation of grape (Vitis vinifera L.) through
cutting. Global Journal of Bio-Science and Biotechnology 9(2): 50-55.
Skillman, J.B., Griffin, K.L., Earll, S. & Kusam, M.
2011. Photosynthetic productivity: Can Plants do Better? InTech. doi:
10.5772/20192
Song, Y.G., Lu, W.P., Wang, J., Shen, Y.J., Wu, Z.S. & Liu, W.D. 2001. Study on promoting the rooting ability of hardwood
cuttings of Amurien grape varieties. China Fruits 1: 4-7.
Spann, T.M. &
Little, H.A. 2011. Applications of a commercial extract of the brown seaweed Ascophyllum
nodosum increases drought tolerance in container-grown ‘Hamlin’ sweet
orange nursery trees. HortScience 46(4): 577-582.
Spinelli, F.,
Fiori, G., Noferini, M., Sprocatti, M. & Costa, G. 2009. Perspectives on
the use of a seaweed extract to moderate the negative effects of alternate
bearing in apple trees. The Journal of Horticultural Science and
Biotechnology 84(6): 131-137.
Srikanth, S., Choong, T.W., Yan,
A., He, J.
& Chen, Z. 2016. An efficient method for adventitious root induction from
stem segments of Brassica species. Front Plant Sci. 7:
943.
Sulichantini, E.D., Sutisna, M.,
Sukartiningsih, S. & Rusdiansyah, R. 2014. Clonal propagation of two clones Eucalyptus pellita F. Muell by mini-cutting. Int. J. Sci. Eng. 6(2): 117-121.
Syõno, K. &
Torrey, J.G. 1976. Identification of cytokinins of root nodules of the garden
pea, Pisum sativum L. Plant Physiology 57(4): 602-606.
Vernieri, P.,
Borghesi, E., Ferrante, A. & Magnani, G. 2005. Application of biostimulants
in floating system for improving rocket quality. Journal of Food
Agriculture and Environment 33(3): 86-88.
Vinodh, S. & Sarmah, D. 2023. Generation of disease-free
propagules of dragon fruit. Quality Transplant Production of Fruit Crops 109.
Whapham, C.A.,
Blunden, G., Jenkins, T. & Hankins, S.D. 1993. Significance of betaines in
the increased chlorophyll content of plants treated with seaweed extract. Journal
of Applied Phycology 5(2): 231-234.
Zhang, Y., Yin,
H., Zhao, X., Wang, W., Du, Y., He, A. & Sun, K. 2014. The promoting
effects of alginate oligosaccharides on root development in Oryza sativa L. mediated by auxin signaling. Carbohydrate Polymers 113:
446-454.
Zodape, S.T. 2001. Seaweeds as a
biofertilizer. J. Sci. Ind. Res. India 60: 378-382.
http://nopr.niscair.res.in/handle/123456789/26485
*Pengarang
untuk surat-menyurat; email: bryl.manigo@usep.edu.ph
|